Check DGV row for missing value with an sql bound back end

I am currently working in vb.net windows express 2013 with an sql back end. I am trying to create an if statement that will look at a specific cell and make a decision based on if the cell has nothing in it or not. I think the return for an sql statement is null but im not 100% sure. I have tried lots of codes I dug up on the internet but none of them have worked for me so far. Here is the line:

Dim Row_Index As Integer = DGVStart.CurrentCell.RowIndex
 If DGVStart.Rows(Row_Index).Cells(1).Value Is Nothing Then

This code does work, as in the windows express is telling me the line is fine, however, it seems that I am always getting the return of nothing, even if something is in the cell.I think this problem has something to do with the way i am pulling the value. I have a datagridview checkbox column which i manually added in the design form and if i change it to cells(0), which is my manually inserted column, it picks it up every time. I need to know how to tell my program to differentiate between is null and is not null.